Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11)
CAT#: TAB-390MZ-S(P)
This novel antibody against ephrin B2 may be used to detect the protein and used as a medicament for inhibiting angiogenesis and lymphangiogenesis in the treatment of diseases in which these processes are implicated, for example, cancer.
Tested Data
Gene Expression
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ELISA
Figure 1 Anti-Human EFNB2 Therapeutic Antibody scFv Fragment (B11) (TAB-390MZ-S(P)) in ELISA
ELISA: Anti-Human EFNB2 Therapeutic Antibody scFv Fragment (B11) [TAB-390MZ-S(P)] detects Recombinant Human Ephrin-B2 Fc
Chimera Protein.
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WB
Figure 2 Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (TAB-390MZ-S(P)) in WB
Western blot analysis of TAB-390MZ-S(P) was performed by with Recombinant Human Ephrin-B2 Fc Chimera Protein.
TAB-390MZ-S(P) incubation concentration: 1.25ng/μL.
The secondary antibody: HRP-Anti-His tag
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
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DB
Figure 3 Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (TAB-390MZ-S(P)) in Dot Blot
DB analysis of TAB-390MZ-S(P) was performed by coating with Recombinant Human Ephrin-B2 Fc Chimera Protein.
The secondary antibody: HRP-Anti-His tag
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/47_A4_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9368/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_5_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_5_6.jpg
Normal Tissue
Figure 9 Skin
Endothelial cells
Staining: Medium
Intensity: Moderate
Fibrohistiocytic cells
Staining: Medium
Intensity: Moderate
Lymphocytes
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_1.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, OE19, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125266-EFNB2
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Specifications
- Host Species
- Human
- Derivation
- Phage M13 library
- Type
- Human antibody
- Specificity
- Human
- Clone
- B11
- Applications
- Enzyme-linked Immunosorbent Assay
- Related Disease
- Cancers such as pancreatic, colon or lung cancer
Target
REVIEWS AND Q&AS
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Customer Review
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Excellent in terms of Cell Lines
Using this antibody on a variety of cell lines produces accurate and clear results. It is a trustworthy reagent for cell culture research.
Extraordinary Supervision
The remarkable efficacy of this antibody demonstrates the effectiveness of quality control procedures. It reliably yields accurate and superior outcomes for various tests.
Easy to measure out
The packaging ensures that the antibody remains stable and sterile for prolonged periods of time by making it easy to aliquot without becoming contaminated. Very useful!
Q&As
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Which typical problems in antibody-based assays should I stay away from?
A: Some common problems include utilizing obsolete or damaged antibodies, diluting them inappropriately, blocking them inadequately, and failing to thoroughly wash them. Following optimum standards can help to alleviate these issues.
-
How can I find out if this antibody responds with other animals?
A: The datasheet often includes information about species cross-reactivity. If the information is unavailable, run validation experiments specific to the species.
-
How can I enhance the binding of my antibody to my antigen?
A: Techniques include prolonging the durations at which antibodies are incubated, changing the pH, ionic strength of buffers and the temperature during incubation.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-390MZ-S(P), RRID: AB_3111924)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone B11"
- CAT
- Product Name
See other products for "EFNB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304 | Recombinant Anti-human EFNB2 Antibody | ELISA, WB, IF, FuncS | IgG |
| HPAB-0164-LSX | Human Anti-EFNB2 Recombinant Antibody (clone A11) | ELISA | Human IgG |
| HPAB-0165-LSX | Human Anti-EFNB2 Recombinant Antibody (clone B1) | ELISA | Human IgG |
| HPAB-0166-LSX | Human Anti-EFNB2 Recombinant Antibody (clone D1) | ELISA | Human IgG |
| MRO-0539-CN | Rabbit Anti-EFNB2 Recombinant Antibody (clone JM53-21) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304-F(E) | Recombinant Anti-human EFNB2 Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-304-F(E) | Recombinant Human Anti-human EFNB2 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| HPAB-0164-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone A11); Fab Fragment | ELISA | Human Fab |
| HPAB-0165-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone B1); Fab Fragment | ELISA | Human Fab |
| HPAB-0166-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone D1); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304-S(P) | Recombinant Anti-human EFNB2 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-304-S(P) | Recombinant Human Anti-human EFNB2 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-0164-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone A11); scFv Fragment | ELISA | Human scFv |
| HPAB-0165-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone B1); scFv Fragment | ELISA | Human scFv |
| HPAB-0166-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone D1); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-389MZ-S(P) | Anti-Human EFNB2 Recombinant Antibody scFv Fragment (2B1) | ELISA | Human antibody |
| TAB-391MZ-S(P) | Anti-Human EFNB2 Recombinant Antibody scFv Fragment (A1) | ELISA | Human antibody |
| TAB-392MZ-S(P) | Anti-Human EFNB2 Recombinant Antibody scFv Fragment (E4 ) | ELISA | Human antibody |
| TAB-393MZ-S(P) | Anti-Human EFNB2 Recombinant Antibody scFv Fragment (TAB-393MZ-S(P)) | IHC, ELISA | Human antibody |
| TAB-394MZ-S(P) | Anti-Human EFNB2 Recombinant Antibody scFv Fragment (TAB-394MZ-S(P)) | IHC, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1100 | Hi-Affi™ Rabbit Anti-EFNB2 Recombinant Antibody (clone DS1100AB) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC163 | Recombinant Anti-EFNB2 Vesicular Antibody, EV Displayed (VS-0425-YC163) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2176 | Anti-EFNB2 Immunohistochemistry Kit | IHC |
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